Gyrolab™ – the nanoliter-scale immunoassay platform

The Gyrolab™ platform uses very precise microfluidic affinity columns. 112 columns are engineered on a single CD. Each column contains streptavidin coated beads which immobilize biotinylated capture reagent for analyte binding. Analyte binding is measured by laser-induced fluorescence detection using Alexa Fluor 647 detection reagents. Automated addition of sample and reagents and an overflow channel with hydrophobic barriers ensure a highly accurate loading of a 15 nL affinity column by a flow-through mechanism. The flow-through mechanism reduces matrix interference, resulting in an increased compatibility with many matrices such as serum, plasma, sputum, cerebrospinal fluid, cell culture supernatant and lysates. Compared to a conventional sandwich ELISA, the Gyrolab™ technology demonstrates an approximately 100x broader dynamic range. The Gyrolab™ platform is ideal for assay miniaturization down to nanoliter scale within a range of 20-1000 nL sample volume. 112 data points on one CD are processed within 1 hour with high sensitivity, accuracy and precision. The flexibility of the Gyrolab™ system allows the development of customized assays. Furthermore, a broad variety of ready to use kits expands the range of applications.